Below is a sample of my Video tutorial on using Visible spectrophtometer for quantification.
- Need to calibrate
- prepare standard calibration curve
- prepare a serial dilution
Sample IA done using Visible Spectrophotometer
How will changing salt concentration affect amylase activity in its hydrolysis of stach into simple sugar measured using visible spectrophotometer by monitoring its blueblack colouration over time.
How will changing temperature affect the activity of invertase enzyme measured using visible spectrophotometer by monitoring the change in absorbance over time.